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Exercise 11 - Exercise 11 Notes Plasmids closed circles of...

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Exercise 11 Notes Plasmids: closed circles of double-stranded DNA, that are capable of moving from one cell to another (or can be induced to do so) o small number of genes Restriction enzymes: bacterial endonucleases that cut DNA at specific sequences of nucleotides; o cut DNA Transformation: When a plasmid vector enters a cell; host cell gains new genes and genetic capabilities Agarose Gel Electrophoresis: used to analyze quality and size of DNA content DNA methylation: when an enzyme called a specific methylase with corresponding specificity to the bacterium’s own restriction enzymes 3 types of restriction endonucleases: o Type II is most widely used recognizes specific, short DNA sequence (4-16 bp) and cuts between specific bases within the restriction site o Eco RI: palindrome; makes staggered cuts ( sticky ends ) o Mg 2+ : stimulates activity of some restriction enzymes o Some restriction enzymes function best in high salt, others require medium levels of
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