exercise 3 - EXERCISE 3: OPTICAL MICROSCOPY GISELLE...

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EXERCISE 3: OPTICAL MICROSCOPY GISELLE ZORNBERG SEPTEMBER 27, 2007
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Disadvantages Brightfield Microscopy Membrane and nucleus are considerably defined Actual color can be observed (cheek cells = mostly clear) Less artifacts and less preparation Not a strong contrast Organelles cannot be observed Lacked detail Brightness of image is falsely perceived Less resolution due to lower refractive indices Darkfield Microscopy Very sharp contrast between object and background Better resolution Details show up well Requires a higher light intensity Does not distinguish actual color of cell Phase Contrast Microscopy Details within cell are much more defined Organelles can be seen Actual color can be observed Sharp contrast between object and background Harder to keep in focus. This is due to the fact that phase contrast purposefully puts light rays out of phase by half a wavelength, with the light from an object to the
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exercise 3 - EXERCISE 3: OPTICAL MICROSCOPY GISELLE...

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