exercise 10 analysis - Exercise 10 Analysis Giselle...

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Exercise 10 Analysis Giselle Zornberg 11-15-07 1. Adding Alkaline SDS causes the cells to lyse because it produces SDS-protein aggregates which become no longer soluble. Placing DNA at alkaline pH (high pH) causes the two polynucleotide strands to be separated. This is known as denaturing the DNA because the normal hydrogen bonding between nitrogen bases is disrupted. Melting, which also occurs, is when the two strands of the double helix become separated. Melting occurs for both plasmid DNA and chromosomal DNA. The difference between plasmid DNA and chromosomal DNA involves the manner in which they conform after abruptly returning to physiological conditions after being melted. 2. After melting at alkaline pH, most plasmids form a native double helix conformation after quickly returning to physiological conditions such as a neutral pH. On the other hand, the hydrogen bonds of chromosomal DNA will rapidly form between complementary bases in short segments of polynucleotide chains after abruptly returning to a neutral pH, causing irregular bonding among polynucleotide chains as well as intramolecular hydrogen bonding along single polynucleotide chains. 3. Ethanol causes salts to go into the aqueous phase, while allowing the plasmid to remain solid, thus causing the plasmid to precipitate out of the solution. This works best at low temperature. 4.
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This lab report was uploaded on 03/20/2008 for the course BIO 205L taught by Professor Hanson during the Fall '07 term at University of Texas at Austin.

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exercise 10 analysis - Exercise 10 Analysis Giselle...

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