exercise 11 analysis - Exercise 11 Analysis Giselle...

Info iconThis preview shows pages 1–2. Sign up to view the full content.

View Full Document Right Arrow Icon
Exercise 11 Analysis Giselle Zornberg 11-29-07 1. A restriction enzyme is a special type of enzyme that cuts a plasmid in just one place. This causes the circular plasmid DNA to open into a linear molecule containing “sticky ends” that are able to join to other pieces of DNA. The DNA sequence that restriction enzyme Eco R I recognizes is: 5’ … G ` AATTC …3’ 3’… CTTAA , G …5’ (The red comma and apostrophe represent where the cuts are) 2. The final contents of the tube containing T white (-) as it was just before being placed in the 37 C water bath for an hour are: 20µL plasmid, 2.5 µL RNA, and 2.5 µL 10X Buffer. The final volume of this tube is 25µL. The final concentration of the buffer is 1X after being diluted. C1 = 10x V1 = 2.5µL C2 = unknown V2 = 25µL C1V1 = C2V2 C2 = (C1V1)/V2 C2 = (10x)(2.5 µL) / 25 µL C2 = 1x 3. The final contents of the tube containing T white (+) as it was just before being placed in the 37 C water bath for an hour are: 18µL plasmid T White, 2µL EcoR I, 2.5µL RNase, and 2.5µL 10X Buffer. The final volume of this tube is 25µL and the final concentration of the buffer is 1X. 4. Transformed bacteria are those bacteria that have incorporated a copy of the plasmid. In order to transform bacteria, a plasmid must first be incubated with restriction enzymes that will open the circular plasmid DNA into a linear molecule containing “sticky ends” that can be joined to other pieces of DNA. The same restriction enzyme is used to cut
Background image of page 1

Info iconThis preview has intentionally blurred sections. Sign up to view the full version.

View Full DocumentRight Arrow Icon
Image of page 2
This is the end of the preview. Sign up to access the rest of the document.

Page1 / 2

exercise 11 analysis - Exercise 11 Analysis Giselle...

This preview shows document pages 1 - 2. Sign up to view the full document.

View Full Document Right Arrow Icon
Ask a homework question - tutors are online