exersie 8 analysis - Exercise 8 Analysis Giselle Zornberg...

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Exercise 8 Analysis Giselle Zornberg 11-01-07 Lab: Thursday 9am-1 1. Glycerol is included as a component of the Gel Loading Buffer because it is a small amino acid that permeates the gel and that, because it is uncharged, does not migrate. Furthermore, because glycerol increases the density of the sample, the sample sinks to the bottom of the well. 2. The top of the gel is negative (the cathode) and the bottom of the gel is positive (the anode). They attract positive and negative proteins, respectively. 3. Water molecules are not precisely aligned in the same orientation all the time while the power is applied because water is polar, with one end composed of a slight negative charge and the other end comprised of a slight positive charge. a. Hydroxyl ion: OH: not drawn towards either wire b. Hydronium ion: H 3 O + : drawn towards the cathode c. Tris + (the buffer): drawn towards the cathode d. Water: not drawn towards either wire e. Chloride ion: Cl - : drawn towards the anode f. NH 3 + -CH
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This lab report was uploaded on 03/20/2008 for the course BIO 205L taught by Professor Hanson during the Fall '07 term at University of Texas at Austin.

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exersie 8 analysis - Exercise 8 Analysis Giselle Zornberg...

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