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Gel Electrophoresis Lab Report.docx - Bad Food at a Good...

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Bad Food at a Good Party Lab ReportDakota KingmaSterrett - 5February 9, 2018
IntroductionUsing a process known as gel electrophoresis, scientists are able to conclude the base lengths of different DNAs. This process consists of placing the same restriction enzymes in all samples of DNA to be observed. Dye is then placed in the solutions, which is then injected into the gel inside the electrophoresis machine. The machine has a positive and negative end, and DNA, being negative, forces its way through the pores further and further away from the positiveside of the gel. The length the DNA travels is based upon the base sizes of the DNA, as the largerthe bases the harder it is for the DNA to squeeze through the pores of the gel. The final distance the DNA travelled is indicated by a dye in the gel that shows itself upon contact with DNA, and these bands can be used to detect similar DNAs in different substances.With this technology, several different samples were taken from a bean dip that been contaminated and resulted in several people becoming sick after a party. The samples of DNA collected were from the entire bean dip, the cheese layer, the sour cream layer, the bean layer, thesalsa layer, and the guacamole layer. Along with these were a MiniOne DNA Marker and 1 Kb DNA Ladder as measuring markers, a positive control, and a Shigella Reference Standard (the bacteria that caused the sickness in the people). Though it is a wild guess, the hypothesis for this lab was that the sour cream was infected with the Shigella bacteria and contaminated the

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Term
Spring
Professor
Mr. Sterrett

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