3When substituting NADPH with DPIP (an electron acceptor that turns from blue to clear as it is chemically reduced) in electron transport, light intensities effect on the rate of the photosynthetic reaction can be observed by recording the absorbance over time. Using aspirated discs cut from spinach leaves, the effect of wavelength on photosynthesis was observed by how many leaves floated when placed in each color light. When testing the absorption of the 4 main pigments involved in the photosynthesis of spinach, the wavelength at which each pigment is most active in photosynthesis is shown. In this lab, the effect of light intensity and light wavelength on photochemical reactions and the activity of photosynthetic pigments were studied. The objective was to see under which conditions photosynthesis occurred the fastest.