Bio Lab 111 Photosynthetic Rates.docx - Everything You Want to Know About Photosynthetic Rates Shelby Moore Bio Lab 11-563 Dr Ben Alexander 2 Abstract

Bio Lab 111 Photosynthetic Rates.docx - Everything You Want...

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Everything You Want toKnow About PhotosyntheticRatesShelby MooreOctober 22, 2015Bio Lab 11-563Dr. Ben Alexander
2AbstractIn this lab, photosynthetic rates were studied in photosynthesis in retrospect to light intensity and wavelength. The activity of pigments at different wavelengths in photosynthesis was also studied. By placing tubes in different light intensities and checking their absorption, it was observed that greater intensity of light accounts for greater photosynthetic rate. When aspirated disks cut from spinach are placed in a beaker of sodium bicarbonate and subject to different colors of light, the amount of disks that float show red and blue wavelengths are most effective for photosynthesis. The best wavelength for photosynthesis is also observed when the 4 main pigments of spinach are tested under different wavelengths to show that they are most effective at 450 nm and 650nm.
3When substituting NADPH with DPIP (an electron acceptor that turns from blue to clear as it is chemically reduced) in electron transport, light intensities effect on the rate of the photosynthetic reaction can be observed by recording the absorbance over time. Using aspirated discs cut from spinach leaves, the effect of wavelength on photosynthesis was observed by how many leaves floated when placed in each color light. When testing the absorption of the 4 main pigments involved in the photosynthesis of spinach, the wavelength at which each pigment is most active in photosynthesis is shown. In this lab, the effect of light intensity and light wavelength on photochemical reactions and the activity of photosynthetic pigments were studied. The objective was to see under which conditions photosynthesis occurred the fastest.

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