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Lab 2 Analysis of DNA and mRNA for MC1R Gene.docx

Lab 2 Analysis of DNA and mRNA for MC1R Gene.docx - Lab 2...

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Lab 2: Analysis of DNA and mRNA for MC1R Gene Background: The genes in DNA encode the protein molecules that are necessary for specific cell functions. There are four nitrogenous bases in DNA: adenine (a), guanine (g), cytosine (c), and thymine (t). The first step in manufacturing the protein is to transfer the information in the double stranded DNA to a single stranded mRNA (messenger RNA). mRNA also has four bases: adenine (a), guanine (g), cytosine (c), and uracil (u). The uracil (u) replaces the thymine (t) in the DNA. In mRNA, the bases are grouped together in triplets called codons. These codons specify the amino acids which are then chained together to manufacture a protein molecule. https://askabiologist.asu.edu/plosable/do-you-have-cavemans-brain The following website has a wealth of information and great illustrations on the translation of DNA to mRNA to Protein. http://www.nature.com/scitable/topicpage/translation-dna-to-mrna-to-protein-393# The MC1R gene encodes the instructions for manufacturing a protein (melanocortin 1 receptor) which is then used by specialized cells to produce melanin, a pigment that gives skin, hair, and eyes their color. In lab today, we are going to analyze the reference gene sequence for the MC1R gene. The reference sequence was down-loaded from the National Center for Biotechnology Information: http://www.ncbi.nlm.nih.gov/ which provides access to biomedical and genomic information. All government funded genetics projects must upload their data to this site. Part A: Counting the Nitrogenous Bases in a DNA Strand
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1. Go to the metasite for Blackboard. Download the MC1R.mat file and the SampleCode.m file. Save both of these to your current folder in MATLAB. 2. For loops can be used to scan through a set of data and count the how many times something occurs. Open up the SampleCode.m file and look at the section of code labeled %% Part A .
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