lab 5 - Procedures Small amounts of four original (Aspirin,...

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Procedures Small amounts of four original (Aspirin, Acetaminophen, Ibuprofen, and Caffeine) analgesics were put into the 4 different test tubes. 1 mL of 50:50 dichloromethane/ethanol was added into each test tube to dissolve the grinded tablets of each analgesic. On the bottom of the TLC plate (about 1 cm from the end), a very thin line was drawn using a pencil. The TLC plate was provided by the TA. Abbreviated names of 4 analgesics were also written, equal distance from each other, leaving 5mm between edges of the TLC plate and the first and last names of analgesics. Ethyl acetate containing 0.5% acetic acid was added into a 150 mL beaker just enough to cover the bottom of the beaker. On the TLC plate, a drop of each analgesic was added on the marked spot right above their name. The TLC plate was inserted into the beaker and closed the top of the beaker with a watch glass to minimize the evaporation (shown above). When the solvent came up to about 1 cm from the top, the TLC plate was removed from the beaker. Using the pencil, the line was drawn across the solvent front. The TLC plate was left in room temperature about 1-2 minutes. Using UV lamp, each dark spot (or analgesics) was circled. The same procedure was repeated again, but this time instead of original analgesics, commercial analgesics (Motrin, Tylenol, Exedrin, Exedrin AF) were used. Distance of the circles from the original analgesics TLC plate and commercial
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lab 5 - Procedures Small amounts of four original (Aspirin,...

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