Notes - Chapter 13 - 070307

Notes - Chapter 13 - 070307 - inserted into the genome 4...

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Chapter 13 – Genomes and Genomics 1. Creating the sequence map of a genome - whole-genome shotgun sequencing - ordered-clone sequencing - draft to finished to complete 2. Bioinformatics – meaning from genomic sequence - annotation - deducing protein-encoding genes from genomic sequence - ORF detection – six possible reading frames - direct evidence from cDNA sequences and ESTs - prediction of binding sites - polypeptide and DNA similarity – BLAST - predictions based on codon bias 3. Structure of the human genome - ~ 45% repetitive – much of it is transposable elements - less than 3% encodes for proteins - number of genes ~25,000 - ~ 19,000 pseudogenes – nonfunctional or inactive - processed pseudogenes – reverse transcribed from RNA and randomly
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Unformatted text preview: inserted into the genome 4. Comparative genomics-homologs – closely related genes-orthologs – genes derived from a common ancestor -paralogs – genes related by duplication in a given organism 5. Functional genomics-global approach to studying the function, expression and interaction of gene products -transcriptome, proteome, interactome-DNA microarrays to study the transcriptome – DNA chips-two-hybrid assay to study the interactome-chromatin immunoprecipitation assay (ChiP) 6. Reverse genetics-through random mutagenesis -through targeted mutagenesis-phenocopying-RNA interference-chemical genetics...
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