Photosynthesis cabbage lab report

Photosynthesis cabbage lab report - Photosynthesis in...

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Photosynthesis in Spinach vs. Purple Cabbage: Which one will photosynthesis more? SEC # 427 TA: Eric Francisco NAME: Sheryl Elizabeth Payne DATE: October 11, 2007 Pledge: I pledge that no unauthorized assistance has been given or received in the completion of this work. Experiments described were preformed by me and/or my lab group and this write-up is entirely my own creative work. Signature ______________________ I. Introduction The sun is the most basic source of energy on earth. Photosynthesis is driven by the sun and occurs in many plants. The resources for photosynthesis are light, water, carbon dioxide, and chloroplasts. The products for photosynthesis are carbohydrates (a sugar) and oxygen; the
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products of photosynthesis are the reactants on cell respiration, which occurs in many other organisms other than plants. The photosynthesis equation is: H 2 O + CO 2 + light CH 2 O + O 2 . The light reactions take place in the thylakoid membrane, which is located in the chloroplasts. The dark reactions, the synthesis of carbohydrates, take place in the stroma fluid inside of the chloroplasts. Robert Hill found that chloroplasts in water can still operate with light and an electron acceptor to release energy and produce oxygen. This showed that the oxygen released in the photosynthesis reaction had to come from the water, not the carbon dioxide. Also, he found that some reactions are light dependant while the synthesis of carbohydrates is light independent. Furthermore he showed that oxidation and reduction initiated by light are an important part of photosynthesis. This demonstrates the conversion of light energy to chemical energy. During the light reactions, electrons are transferred to higher energy levels when the leaf absorbs light. Electron-acceptor molecules are alternately being oxidized and reduced. Electrons reduce NADP + to form NADPH (a reactant use in the dark reactions). To detect this process a dye, DPIP is added and the more photosynthesis that takes place, the more the DPIP changes color from blue to clear and the more photosynthesis is occurring. A spectrophotometer is used to measure the loss of color. The DPIP is reduced, thus it changes color. If something is reduced, that means it accepts electrons. The Hill Reaction chemical equations are: 2DPIP + 2H 2 O + Chloroplasts + light 2DPIPH 2 + O 2 and DPIP + e - DPIP (colorless). We chose to compare the rate of photosynthesis in (green) spinach and purple cabbage. Chlorophylls are the greenish pigments in plant’s photosynthetic tissues and they capture light used in photosynthesis. We believe that the green spinach contains more chlorophylls than the purple cabbage and thus the spinach will photosynthesis more. Our hypothesis states that the green spinach will photosynthesize at a greater rate than the purple cabbage thus producing a
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higher transmittance percentage. The independent variable is the type of chloroplast (spinach or purple cabbage) and the dependant variable is the percent transmittance. II. Materials and Methods
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Photosynthesis cabbage lab report - Photosynthesis in...

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