Lab report 3 2003 - Lab Report 3 Techniques of Microscopy II Fluorescence Christopher Chu Wednesday 6:00pm 10:00pm Objectives 1 To learn how to

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Lab Report 3 Techniques of Microscopy II Fluorescence Christopher Chu Wednesday 6:00pm – 10:00pm Objectives: 1) To learn how to apply different stains and interpret those stains in a fluorescent microscope. 2) To apply knowledge of fluorescence to manipulate microscope images on the computer. 3) To be able to interpret the different stages of mitosis under different types of microscopes. Materials: DAPI, fluorescein, rhodamine, microscopes, computers, microscope slides, PBS, propidium iodide, pasteur pipette, BSA, coverslips, L8 cells, cheek cells, commercially prepared slides, and methylene blue. Procedure: Begin by setting up the experiment for C3 or C4. This involves beginning the incubation of the petri dish after the slides have been rinsed with PBS. Then start observing fluorescent beads under a fluorescent microscope. This is to observe the difference in the observable size of the beads versus the actual size of the beads based on the distance in Abbe's question. Next, observe cheek epithelial cells using fluorescence microscopes, using Propidium Iodide and DAPI as nuclear stains. Both stain DNA but emit different wavelengths of light. Set the epithelial cells on
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This note was uploaded on 04/07/2009 for the course BIO 206L 49125 taught by Professor Dr.allenlloyd during the Spring '09 term at UT Arlington.

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Lab report 3 2003 - Lab Report 3 Techniques of Microscopy II Fluorescence Christopher Chu Wednesday 6:00pm 10:00pm Objectives 1 To learn how to

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