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bio 125 bacterial growth lab report 1.docx

bio 125 bacterial growth lab report 1.docx - Kyle Colyn Dr...

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Kyle Colyn Dr. Eppinga 9-17-16 Lab Report Microbial Growth Abstract This week’s experiment consisted of two substances that inhibit microbial growth being compared by culturing p. aeruginosa bacteria on three separate nutrient agar plates. We added inhibitors to determine the zone of inhibition for each of the substances. First, a premade broth with bacteria in it was used to swab the plates. We then proceeded to add molasses and an antiseptic solution to one plate each before the incubation. The substances were added by soaking a paper disk in each of the substances and then setting it on the nutrient agar plate. They plates were then cultured for 28 hours at 37 degrees Celsius. We then recorded the zones of inhibition. The first plate, containing no solution, grew bacteria across the entire surface. The plate with molasses grew bacteria across the surface, but not around the .5 cm diameter disk. The plate with antiseptic was similar to molasses as it grew bacteria across the surface, but not around the 2 cm diameter disk. Zone of inhibition was measured as 2 cm and .5 cm in diameter for the antiseptic and molasses. Introduction Last week we experimented with microbes and we discovered that they are basically unavoidable because they are on nearly every surface we touch. Not all microbes are harmful, but those that are can cause serious problems such as the flue or infections. Sanitation in therefore very important to the health of our bodies.
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