BIO f325 Summer 2007 - II Exam - key only

BIO f325 Summer 2007 - II Exam - key only - GENETICS (BIO...

Info iconThis preview shows pages 1–2. Sign up to view the full content.

View Full Document Right Arrow Icon
GENETICS (BIO f325) SUMMER 2007 II Exam- Key 06/25/07 Total points: 120 _______________________________________________________________________ _ 1. In the cross, Hfr (pro + ) x F - (pro - ), the F plasmid is excised imprecisely from the Hfr chromosome in the first step. The excised F carries pro + gene of the donor chromosome, resulting in F’pro + . This F’ carrying the pro + gene is next transferred to the F - (pro - ) cells early in the mating process. Cells carrying an F’pro + , when used in further matings, convert a majority of the F - (pro - ) cells to F’pro + cells. 2. (i) arg-bio-leu (ii) arg-bio = 12.76 mu bio-leu = 2.12 mu 3. (c) 4. If gene transfer occurs by transformation, no DNA would be transferred from the ampicillin resistant strain to the ampicillin sensitive strain in the presence of DNase, as the donor DNA would be degraded. If gene transfer occurs by transduction, presence of DNase in the medium would have no effect on the transfer of the ampicillin resistance gene from the resistant to the sensitive strain. 5.
Background image of page 1

Info iconThis preview has intentionally blurred sections. Sign up to view the full version.

View Full DocumentRight Arrow Icon
Image of page 2
This is the end of the preview. Sign up to access the rest of the document.

Page1 / 3

BIO f325 Summer 2007 - II Exam - key only - GENETICS (BIO...

This preview shows document pages 1 - 2. Sign up to view the full document.

View Full Document Right Arrow Icon
Ask a homework question - tutors are online