Key+Practice+Exam+2

Key+Practice+Exam+2 - BIOL2800 Summer 2004 Exam 2 Name: _ 1...

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BIOL2800 Name: _______________________________ 1 Summer 2004 Exam 2 Please answer all of the following questions as clearly and concisely as possible. Please follow all of the directions or you may lose points. PLEASE WRITE YOUR NAME ON ALL OF THE PAGES!!! 1. ( 2 pts. each) Matching. Write the LETTER of the BEST answer (only one best answer) for each statement. Each letter may be used more than once or not at all. A. Sigma factor B. Hydrogen bond C. Rho protein D. RNA polymerase E. AT-rich sequence F. TBP G. Peptide bond H. cAMP I. UTP J. Termination sequence K. Amino L. Glycine M. None of the answers is correct. ____E_____ is located at about –10 in a bacterial promoter ____A_____ helps the bacterial RNA polymerase bind appropriately to a promoter ____D_____ adds one nucleotide at a time using the DNA as a template ____M_____ the type of bond formed between successive nucleotides in an mRNA strand ____C_____ is a protein that can be used to terminate transcription ____I_____ is a nucleotide that can be incorporated into a growing mRNA strand ____B_____ the type of bond that that holds two nucleic acid strands together 2. The rate of transcription of a gene can be controlled by multiple mechanisms. Write a brief explanation for each question below. a. (3 pts) Briefly explain how a transcription factor can decrease the rate of transcription of a gene. TFs can decrease transcription levels by blocking RNA polymerase from binding to the gene and/or tightening the DNA so that the RNA polymerase cannot access the template sequence. b. (3 pts) Describe one other way in which transcription of a gene can be downregulated. a mutation in the promoter sequence so that the TF can’t bind well OR a mutation in the TF so that its
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Key+Practice+Exam+2 - BIOL2800 Summer 2004 Exam 2 Name: _ 1...

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