Determing water potential of potato tuber cells.docx

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Running Head: WATER POTENTIAL OF POTATO TUBER CELLS 1 DETERMINING WATER POTENTIAL OF POTATO TUBER CELLS Name Institutional Affiliations
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WATER POTENTIAL OF POTATO TUBER CELLS 2 Table of Contents Aim ....................................................................................................................................................... 3 Introduction ......................................................................................................................................... 3 Theory ............................................................................................................................................... 3 Methods and Equipment ..................................................................................................................... 6 Equipment ......................................................................................................................................... 6 Method ............................................................................................................................................ 12 Results ................................................................................................................................................ 13 Discussion ........................................................................................................................................... 14 Conclusion .......................................................................................................................................... 15 References .......................................................................................................................................... 16
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WATER POTENTIAL OF POTATO TUBER CELLS 3 Aim The primary aim of this laboratory practical was to do an experiment by producing a series of dilute solutions to develop a calibration curve for identifying water potential of plant tissue. This objective was met by using potato tuber cells that were subjected to solutions of different solute concentrations for determination of their water potential. Introduction Water movement in and out of plant tissue plays important roles in cell physiology. Besides these functions, most research work has proved that water forms the greatest mass of plant tissue compared to the dry matter. In fact, the cytoplasm of a cell is mainly composed of water with dissolved salts. Therefore, the subject of water potential is very important in understanding water movement in the plant tissue[Dav09]. Some roles of water in tissue cells include maintenance of cell rigidity to ensure robust physical support to the plant. Although there are specialized cells that enhance strength for a plant’s structure, water intake into the cells have proven to add physical strength to the plant tissue by making the cells turgid. Water is also used in the transportation of nutrients within the tissue of the plant. Plants take in water through the roots and used for photosynthesis as a raw material. Lastly, temperature regulation is carried out using water by the plant[Dav09]. The movement of water across the cells within the plant tissue occurs through a process known as osmosis. Osmosis involves the movement of water molecules where these molecules are concentrated (dilute solution) to where the water molecules are scarce (concentrated solution) across a molecule selective membrane. Therefore, osmosis can be seen to depend on the concentration of a solution[Ste09]. In this experiment, we determine water potential of plant tissue using a series of solutions with known concentrations. In this particular biology practical, a series of solutions were prepared from 1.0 molar of sucrose solution while the plant tissue used was potato tuber cells. This study is relevant to the study of biology regarding plant cell physiology. Besides the physiology, the knowledge of preparing solutions is also gained thus contributing to overall success in understanding water intake and expulsion in plant tissue. Theory As learned in the previous section, osmosis is useful in water uptake by plant tissues. This movement occurs when there is a potential existing between two or more cells. This potential is known as water potential and is defined as the measure of the tendency of water molecules to move from the one solution into another solution of different concentration. This definition
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