Armanious, M-Homework Module 6.pdf

Armanious, M-Homework Module 6.pdf - Matthew Armanious Cell...

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Matthew Armanious Cell and Tissue Engineering Homework, Module 6 1) b) Since you end up using a ratio between N and N o , and since the number will still triple, the rate constant for cell proliferation will remain the same, and the time will also remain the same at 32.5 hours . c) The answer in b) can become invalid if the environment the cells are in changes so that the rate of growth isn’t constant. The rate of growth can be effected by cell density, nutrient availability, and waste product accumulation. For instance, if the confluency in a petri dish is close to 100%, the cells won’t be able to multiply as quickly as initially capable. ( Tissue Engineering, Saltzman, pg 102)
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3) Cells that are cultured outside the body will only grow after numerous conditions are established. Initially, according to Tissue Engineering, Saltzman, pg 97 , isolated liver cells are actually very difficult to grow in culture. Not only are there conditions that are
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