Armanious, Matt-Module 9 Homework.pdf

Armanious, Matt-Module 9 Homework.pdf - Matthew Armanious...

Info icon This preview shows pages 1–2. Sign up to view the full content.

View Full Document Right Arrow Icon
Matthew Armanious Cell and Tissue Enginering Module 9 Homework 1. Understanding and analyzing molecular transport mechanism is necessary when designing basic cell culture experiments and perfusion of biomaterials using a bioreactor. Oxygen delivery to cells in culture can by modeled using Fick’s Law. Transport of oxygen from the gas phase (Pg) to the cell phase (Pc) is driven by the oxygen gradient across the height of the medium. The rate of oxygen uptake by cells however can be modeled with Michaelis-Menten kinetics (what we use for facilitated diffusion) (from Pc into the cell). a) In lecture, we graphed dn/dt as a function of dC/dx for simple diffusion. What is the slope of this line? What do increases or decreases in the slope mean biologically? The slope of this line is the "#$% &’ ()’’*+)&, -&,.%,$/#$)&, 0/#1)%,$ . When this slope increases, that means the rate of diffusion increases as well. This happens when the concentration of molecules outside the cell is very high relative to the internal cell concentration. When the internal and external concentrations are similar (a low concentration gradient), the rate of diffusion will be lowered which also decreases the slope. b) Now assume the concentration gradient is a constant. How does the rate of diffusion (dn/dt) change with the surface area of the cell and the permeability of the diffusing molecule? Graph dn/dt as a function of A or P and describe the function.
Image of page 1

Info icon This preview has intentionally blurred sections. Sign up to view the full version.

View Full Document Right Arrow Icon
Image of page 2
This is the end of the preview. Sign up to access the rest of the document.

{[ snackBarMessage ]}

0/0 PREVIEWS LEFT
Sign up to access 24/7 study resources for your classes

What students are saying

  • Left Quote Icon

    As a current student on this bumpy collegiate pathway, I stumbled upon Course Hero, where I can find study resources for nearly all my courses, get online help from tutors 24/7, and even share my old projects, papers, and lecture notes with other students.

    Student Picture

    Kiran Temple University Fox School of Business ‘17, Course Hero Intern

  • Left Quote Icon

    I cannot even describe how much Course Hero helped me this summer. It’s truly become something I can always rely on and help me. In the end, I was not only able to survive summer classes, but I was able to thrive thanks to Course Hero.

    Student Picture

    Dana University of Pennsylvania ‘17, Course Hero Intern

  • Left Quote Icon

    The ability to access any university’s resources through Course Hero proved invaluable in my case. I was behind on Tulane coursework and actually used UCLA’s materials to help me move forward and get everything together on time.

    Student Picture

    Jill Tulane University ‘16, Course Hero Intern