Lab 9 Cultural and Physiological Characteristics of Bacteria II - Student.pptx

Lab 9 Cultural and Physiological Characteristics of Bacteria II - Student.pptx

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CHARACTERISTICS OF BACTERIA Part II
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Day 1
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Preparation You will be continuing to use the following bacteria: P. aeruginosa, S. aureus, E. coli, E. aerogenes, P. vulgaris, B. cereus,, L. lactis and one “unknown” Use the TSA Slant you saved from Lab # 8 Bacteria will be inoculated into their own independent tube of the following media: Nitrate Broth Urea Gelatin MR-VP Phenylalanine Broth Simmons Citrate Tryptone Broth SIM Oxidase Catalase
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Day 2
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Fermentation vs Respiration Conversion of organic molecules to an energy source such as ATP. Glucose turns into pyruvate via glycolysis. What happens next depends on what catabolic system is used
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Fermentation Final electron acceptor is an organic molecule Does not produce as much energy as respiration Pyruvate → General end products are organic acids and gases (CO 2 , H 2 …) Mixed acid fermentation 2,3-butanediol fermentation Can also be used as a general term for the breakdown of polysaccharides into monomers that can then be fermented Ex: lactose → glucose & galactose → pyruvate
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Respiration Final electron acceptor is an inorganic molecule Oxygen → aerobic respiration Nitrate, sulfate… → anaerobic respiration General pathway for respiration Glycolysis → Kreb’s cycle → electron transport chain What is the ETC? See flow chart. Transfer of electrons to molecules with a more positive reduction potential. We are indirectly testing for the presence of an ETC! Flavoprotein FeS Cluster Ubiquinone Cytochrome C Cytochrome C Oxidase Final Electron Acceptor
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Catalase Test Flavoproteins can pass electrons on oxygen directly This leads to the production of two highly potent cellular toxins Superoxide radicals Hydrogen peroxide (H 2 O 2 ) Some bacteria have enzymes capable of breaking down these toxins ( aerobic & facultative anaerobes ) Superoxide dismutase (SOD) Catalase Flavoproteins O 2 Toxins -
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Catalase Test Differential test Detects catalase Converts hydrogen peroxide → water and oxygen Balanced equation: 2H 2
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