{[ promptMessage ]}

Bookmark it

{[ promptMessage ]}

Lecture30-BIOL201-204-U5-Glycolysis Part 2_post-class.pdf

Lecture30-BIOL201-204-U5-Glycolysis Part 2_post-class.pdf -...

Info icon This preview shows pages 1–4. Sign up to view the full content.

View Full Document Right Arrow Icon
Metabolism Part I Universal metabolic pathways Nucleotides Unit 5 Metabolism IMPORTANT NOTE: As preparation for the final exam, you should bring a copy of the EXAM II Reference Material to EVERY CLASS from now on. You will need it!!
Image of page 1

Info icon This preview has intentionally blurred sections. Sign up to view the full version.

View Full Document Right Arrow Icon
Metabolism I Learning Outcomes Week 12-13 Predict the organic chemistry of the oxidation-reduction and phosphorylation reactions of anaerobic Glycolysis focusing on nucleophile and leaving groups without the reference to the detailed mechanisms the enzymes use to speed-up these reactions. Critically analyze Glycolysis (including alcohol and lactic acid fermentation) and the Tricarboxylic Acid (TCA) Cycle, and identify the following, based on the chemical information in the reactions: The type of reaction being catalyzed (i.e. oxidation-reduction reactions, phosphorylation reactions, carbonyl modification reactions, C-C bond cleavage and formation reactions) Important nucleophiles, electrophilic centres and leaving groups. The class of enzyme that is catalyzing the reaction (based on the first level of the international classification system established by the IUBMB) The need for any additional co-factors and/ or reactants, and what they would be Any high-energy intermediates (HEIs) that are produced (enzyme-bound and/or free) The products of importance (i.e. ATP, NADH, etc.) Apply the same strategy of critical analysis of chemical reactions to new, potentially unknown metabolic pathways (e.g. the pentose phosphate pathway), based what you have learned from your analysis of glycolysis and the TCA cycle. Identify any reactions that appear to be identical to reactions in other pathways you know. For Glycolysis: Explain the biochemical purpose of the two anaerobic Glycolysis reactions discussed, in cells/organisms that ‘use’ these reactions. Explain how the electrons from NADH produced in the cytosol during aerobic Glycolysis reach the matrix of the mitochondrion. Describe the structure of the pyruvate dehydrogenase (PDH) complex including all important co-factors/ prosthetic groups, and explain how they aid in the reaction mechanism. Distinguish between aerobic catabolism and an aerobic catabolism as they relate to the process of respiration, fermentation, inputs, outputs and the fates of pyruvate and NADH. Relate this to the final energetic output of each one. Demonstrate understanding of this unit by successfully solving questions of the types in Problem Set #8 . 2
Image of page 2
3 Most enzymes encountered in BIOL201 can be categorized into one of the following classes of enzymes: 1.Oxidoreductase: catalyzes an oxidation-reduction reaction between two molecules. Subclass: Dehydrogenase: catalyzes a redox reaction involving 2 e- and 1 or 2 H + between 2 molecules, one of which is NAD(P)H/NAD(P) + /FADH 2 / FMN 2.Transferase: catalyzes the transfer of functional groups between molecules.
Image of page 3

Info icon This preview has intentionally blurred sections. Sign up to view the full version.

View Full Document Right Arrow Icon
Image of page 4
This is the end of the preview. Sign up to access the rest of the document.

{[ snackBarMessage ]}

What students are saying

  • Left Quote Icon

    As a current student on this bumpy collegiate pathway, I stumbled upon Course Hero, where I can find study resources for nearly all my courses, get online help from tutors 24/7, and even share my old projects, papers, and lecture notes with other students.

    Student Picture

    Kiran Temple University Fox School of Business ‘17, Course Hero Intern

  • Left Quote Icon

    I cannot even describe how much Course Hero helped me this summer. It’s truly become something I can always rely on and help me. In the end, I was not only able to survive summer classes, but I was able to thrive thanks to Course Hero.

    Student Picture

    Dana University of Pennsylvania ‘17, Course Hero Intern

  • Left Quote Icon

    The ability to access any university’s resources through Course Hero proved invaluable in my case. I was behind on Tulane coursework and actually used UCLA’s materials to help me move forward and get everything together on time.

    Student Picture

    Jill Tulane University ‘16, Course Hero Intern