abstract four.docx - Fluorescent Protein-Based...

Info icon This preview shows page 1. Sign up to view the full content.

View Full Document Right Arrow Icon
Fluorescent, Protein-Based Nanoparticles with Dimensions Controlled on Atomic Resolution Dr. Challa Kumar Department of Chemistry & MCB University of Connecticut, Storrs, CT 06269 Background : Quantum dots are nanoscale particles of semiconducting material that can be embedded in cells for various experimental purposes, such as labeling proteins. However, quantum dots are toxic, high priced, challenging to make and have low biocompatibility. Studies were conducted on how to make protein-based nanoparticles that are biodegradable, sustainable and bioabsorbale to replace quantum dots. Methods and Results : The model protein that this study used as a starting point for nanoparticle formation included Bovine serum albumin (BSA), which is abundant, versatile and inexpensive. The method used performed controlled crosslinking of the protein, used a chemical agent to crosslink the amine and carboxyl groups, thermally equilibrated the mixture to achieve precise particle size and then labeled the protein with the appropriate fluorophore. This study resulted in the creation of the FluoDot24-N. A
Image of page 1
This is the end of the preview. Sign up to access the rest of the document.

{[ snackBarMessage ]}

What students are saying

  • Left Quote Icon

    As a current student on this bumpy collegiate pathway, I stumbled upon Course Hero, where I can find study resources for nearly all my courses, get online help from tutors 24/7, and even share my old projects, papers, and lecture notes with other students.

    Student Picture

    Kiran Temple University Fox School of Business ‘17, Course Hero Intern

  • Left Quote Icon

    I cannot even describe how much Course Hero helped me this summer. It’s truly become something I can always rely on and help me. In the end, I was not only able to survive summer classes, but I was able to thrive thanks to Course Hero.

    Student Picture

    Dana University of Pennsylvania ‘17, Course Hero Intern

  • Left Quote Icon

    The ability to access any university’s resources through Course Hero proved invaluable in my case. I was behind on Tulane coursework and actually used UCLA’s materials to help me move forward and get everything together on time.

    Student Picture

    Jill Tulane University ‘16, Course Hero Intern