BIOL 1210 Cut It Out CRISPR Case Study.docx

BIOL 1210 Cut It Out CRISPR Case Study.docx - BIL 1210 001...

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BIL 1210 001 For the case study, “Cut it out! Editing DNA with CRISPR-Cas9,” answer the questions in this Word document while you read the PDF document. Do one part at a time. * Qs are 0.5 bonus each Also refer to the Zhang article, “Development and applications of CRISPR-Cas9 for genome engineering,” and the Nelson article, “In vivo genome editing improves muscle function in a mouse model of Duchenne muscular dystrophy.” Part I 1. Where did the CRISPR system first originate in nature and what is its purpose in that context? 2. How is Cas9 able to bind to specific sequences of DNA? What does Cas9 do to the sequences it interacts with? 3. What advantage does the CRISPR-Cas9 system offer compared to previous genome editing technologies? 4. David remembers one of his friends discussing how the government should focus funding more on clinical research rather than basic research. His friend felt that basic research in model organisms was not very important anymore since we know so much about genetics. Why are model systems an important part of research? What might David
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