Cell Bio- Cell counting and Cell Growth.ppt

Cell Bio- Cell counting and Cell Growth.ppt - CELL COUNTING...

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CELL COUNTING CELL GROWTH
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Cell Counts by Hemocytometer
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Hemocytometer method of cell counting : The hemocytometer cell counting method uses a specially crafted gridded-glass slide consisting of two chambers, each divided into nine 1 x 1 mm squares. A glass cover is supported by the grid, and the cell suspension is loaded via capillary action into the chamber. The cells lie over the grid occupying a volume of 0.1 μL for each 1 x 1 mm square, and are counted manually. The number of cells counted in a specific number of squares and the dilution factor are used to calculate the original concentration in cells/mL.
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Table 1—Cell lines Animal Organ HEK-293 ATCC CRL-1573 Human Kidney A431 ATCC CRL-2592 Human Skin CHO-M1WT2 ATCC CRL-1984 Chinese hamster Ovary CHSE ATCC CRL-1681 Chinook salmon Embryo COLO-205 ATCC CCL-243 Human Colon COS-7 ATCC CRK-1651 African monkey Kidney HeLa ATCC CCL-2 Human Cervix HepG2 ATCC CRL-10741 Human Liver HL-60 ATCC CCL-240 Human Blood J774A.1 ATCC TIB-67 Mouse Blood Jurkat ATCC TIB-152 Human Blood MCF7 ATCC HTB-22 Human Breast MRC-5 ATCC CCL-171 Human Lung NIH/3T3 ATCC CRL-1658 Mouse Embryo PC-12 ATCC CRL-1721 Rat Adrenal gland SF-21 Invitrogen 12682-019 Insect Ovary U266 ATCC TIB-196 Human Blood U-2 OS ATCC HTB-96 Human Bone K562 ATCC CCL-243 Human Bone marrow Invitrogen R7788-110 Human Adipose-derived stem cells Human aortic smooth muscle cells Human Smooth muscle Human pulmonary artery endothelial cells Human Blood vessel Human pulmonary artery smooth muscle Human Smooth muscle Human umbilical vein endothelial cells Human Blood vessel
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WHAT CAN AFFECT YOUR COUNT? WHAT CAN AFFECT YOUR COUNT? Proper sample preparation technique is critical when working with the traditional hemocytometer. The glass hemocytometer, with its free cover slip, is subject to over-filling , resulting in cell numbers that are often higher than those produced by disposable, fixed cover slip hemocytometers.
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