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ePracbook_Algae in our waterways.docx

ePracbook_Algae in our waterways.docx - BIOL116 Practical...

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BIOL116 Practical 11: How much algae is there in our waterways? Objectives Skills * Understand how we can use standard curves to quantify unknown quantities of a variable. * Use Excel to plot values on a scatterplot and generate a formula that describes a linear line-of-best-fit. * Use correct micropipetting technique to create serial dilutions from a solution of known concentration. * Calculate the concentration of serial dilutions made from a solution of know concentration. * Understand how a spectrophotometer works, and how changes in concentration of a coloured solution is related to absorbance. * Measure the absorbance of solutions of known concentration to make a standard curve, and use this standard curve to calculate the concentration of a solution where the concentration is unknown. Biological concepts * Blue-green algae can change in concentration in streams, often in response to nutrient influx in waterways. High concentrations of blue-green algae can have negative health and ecological consequences on the waterway. Note: Text coloured blue are sections which we expect you to fill in, or provide an answer. These blue sections may be assessed for completeness when your tutors mark your ePracbooks. Text coloured green are sections where you need to insert or paste content into your ePracbook. These green sections may be assessed for completeness when your tutors mark your ePracbooks. Text coloured purple are sections that we recommend you fill in for your own learning. We strongly encourage you to complete these sections, but they will NOT be assessed when your tutors mark your ePracbooks Exercise 1: How to read a standard curve. Your tutor will demonstrate to you how to use a standard curve.
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