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Lab 5 Pre-Lab.docx

Lab 5 Pre-Lab.docx - Thin Layer Chromatography...

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Thin Layer Chromatography: Identification of Unknown Mixtures Introduction: This experiment is used to determine the components in several mixtures using thin layer chromatography. This is a type of solid/liquid chromatography that allows the comparison of different substances based on their individual absorbance to the silica gel of the plate. All of the unknown mixtures are dissolved in an equal ratio solution of methylene chloride and ethanol. Once the compounds are spotted and placed in the developing chamber, made of a large beaker, watch glass, and a piece of filter paper, holds a small volume of ethyl acetate with acetic acid. The chamber becomes filled with vapors of this solvent and it is the solvent that is greatly responsible for the absorbance and the distance covered by the compound on the plate. UV lights allow for the visualization of the spots once removed from the chamber and this allows for two measurements: the distance covered by the compound, and the distance travelled by the solvent. These measurements create a ratio known as the retention factor (R f ) which can help determine if two unknown compounds are the same substance. In the lab, the R f values can allow the identification of the commercial names of the six unknown mixtures based off the information provided in the mixture composition table below. Table of Reagents: Mixture Composition Table Analgesic Mixture Known Components Anastasia’s Analgesic Elixir Acetaminophen Acetylsalicylic Acid
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