Exam 3 Cheat Sheet.docx

Exam 3 Cheat Sheet.docx - Overview of Transcription E coli...

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Overview of Transcription; E. coli RNA Polymerases Know the general features of transcription o Enzyme involved (RNA polymerase) Know the subunits Know the difference between the core and holoenzyme Which subunit recognizes the promoter o Substrates o Mg 2+ required o Which strand read and direction of synthesis o De novo synthesis o What is the promoter Review Exercises Recommended: 1, 2, 3, 4, 5 E. coli Promoters Know the common features of E. coli promoters o -10 region (Pribnow box) o -35 region o TSS (transcription start site o Know the difference between the core and the extended promoter Review Exercises Recommended: 6, 7 Steps of Prokaryotic Transcription Chain initiation o Know the difference between the closed and open complex o Know the usual start nucleotide (purine) o Just a few nt added before subunit released Chain elongation o Movement of transcription bubble o Role of topoisomerase o How RNA polymerase launches itself from promoter Chain termination o Know the principles of intrinsic termination Hairpin loop followed by string of U residues o Know the principles of rho termination Hairpin loop causes RNA polymerase to pause Rho protein catches up to polymerase and forces dissociation Review Exercises Recommended: 8 Regulation of Transcription: Sigma Factors and Enhancers Know that there are multiple sigma factors 1
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o Know the significance of this in terms of normal cellular function o Know the significance of this in terms of viral infection Know what enhancers are where they’re usually located o Recognized by transcription factors o
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