C 318 Exam 3 Nov 29, 2017 Name: 1. (5pts total) Give the full name and/define the following: a) QQQ triple quad, quadrupole-quadrupole-quadrupole b) EI electron impact, electron ionization, electron impact ionization c) Q-TOF quadrupole time of flight d) MALDI matrix assisted laser desorption ionization e) ESI electrospray ionization f) TOF time of flight g) Hard ionization ionization method which imparts a lot of energy into molecule to form some molecular (intact) ion(s) + mostly fragments, ionization method that induces fragmentation h) Soft ionization ionization method which imparts very little energy into molecule to form mostly molecular (intact) ion(s), ionization producing only/mainly intact ions i) Ionization energy amount of energy required to remove an electron from the ground state, amount of energy required to remove an electron j) MS-MS tandem mass spectrometry, Mass spectrometry-mass spectrometry. Two mass analyzers coupled in sequence (AJ 2016) Coupling of two mass analyzers to separate intact ions and subsequently generated fragments in 1 st and 2 nd mass analysis stages, respectively 2.(2pts total) a) Who discovered electrons and built the first mass spectrometer?JJ Thompson b) Who invented a method of producing multiply charged protein ions from a spray? 3. (2 pts ea., 4 total) Define the following. Use equations if appropriate. a. Chromatographic resolution R = 2(X1 – X2) /(W1+W2) a measure of how well separated two peaks are from one another in a spectrum b. Mass spectrometric resolving power R = m/Δm, a measure of how sharp a peak is. Δm at fwhm 4. (4pts) Draw a block diagram of a mass spectrometer. Label all components.
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- Spring '18
- Mass Spectrometry, mass spectrometer, Electrospray ionization, pts extra credit, ionization method, ESI electrospray ionization