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Class 9 Srcprotein

Class 9 Srcprotein - Src the protein Reading The Hunting of...

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        Src, the protein Reading:   The Hunting of the Src G. Steve Martin , Nature Reviews|Molecular Cell Biology 2001 2:467-475
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Fusiform mutants of RSV Cell transfor mation in culture i.d. of v- Src protein Genetic i.d. of v- src gene I.D. of SH3 domain Ras/Src signallin g Src k.o. Src mutations in colon cancer Disc. of RSV Focus assay for RSV Rep.- defective RSV c-src proto- oncogen e Src is a protein tyr kinase I.D. of SH2 domain SH2 domains bind P-Tyr Crystal structure of Src History of Src By 1977: an emerging concept of what oncogenes were , but little  insight into what they were doing.
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 “Fusiform” mutants of RSV: a  derivative of RSV that induces a  fusiform phenotype, as opposed to the  transformed phenotype seen with wt  (i.e., 1909) RSV. Fusiform:  spindled, elongated
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The genes: c-src, v-src
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The c-Src and v-Src Proteins SH1 domain SH domains:   S rc  H omology domains M: membrane, U: unique, L: linker, R: regulatory *:  positions of mutations in v-Src relative to c-Src P :Primary phosphorylation sites in vivo
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v-src differs from c-src: ∙ by carboxyl-terminal deletion and substitution ∙ several point mutations ∙ sites of phosphorylation Do they have the same biological activities? • the cloned v-src gene transforms cells • c-src has low transforming ability
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Src is “myristylated” at its amino terminus • covalent attachment of myristylate (C14 fatty acid) • the lipid anchors Src to the plasma membrane Plasma membrane inside outside
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Brugge and Erikson, 1977: • Induced tumors in mice with an RSV derivative that was able to infect mammalian  cells  • They expected the mice would make antibodies to v-Src •  Isolated an antibody that could immunoprecipitate a 60 kD protein expressed only in 
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Class 9 Srcprotein - Src the protein Reading The Hunting of...

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