Class 9 Srcprotein - Src, the protein Reading: The Hunting...

Info iconThis preview shows pages 1–9. Sign up to view the full content.

View Full Document Right Arrow Icon
        Src, the protein Reading:   The Hunting of the Src G. Steve Martin , Nature Reviews|Molecular Cell Biology 2001 2:467-475
Background image of page 1

Info iconThis preview has intentionally blurred sections. Sign up to view the full version.

View Full DocumentRight Arrow Icon
Fusiform mutants of RSV Cell transfor mation in culture i.d. of v- Src protein Genetic i.d. of v- src gene I.D. of SH3 domain Ras/Src signallin g Src k.o. Src mutations in colon cancer Disc. of RSV Focus assay for RSV Rep.- defective RSV c-src proto- oncogen e Src is a protein tyr kinase I.D. of SH2 domain SH2 domains bind P-Tyr Crystal structure of Src History of Src By 1977: an emerging concept of what oncogenes were , but little  insight into what they were doing.
Background image of page 2
 “Fusiform” mutants of RSV: a  derivative of RSV that induces a  fusiform phenotype, as opposed to the  transformed phenotype seen with wt  (i.e., 1909) RSV. Fusiform:  spindled, elongated
Background image of page 3

Info iconThis preview has intentionally blurred sections. Sign up to view the full version.

View Full DocumentRight Arrow Icon
The genes: c-src, v-src
Background image of page 4
The c-Src and v-Src Proteins SH1 domain SH domains:   S rc  H omology domains M: membrane, U: unique, L: linker, R: regulatory *:  positions of mutations in v-Src relative to c-Src P :Primary phosphorylation sites in vivo
Background image of page 5

Info iconThis preview has intentionally blurred sections. Sign up to view the full version.

View Full DocumentRight Arrow Icon
v-src differs from c-src: ∙ by carboxyl-terminal deletion and substitution ∙ several point mutations ∙ sites of phosphorylation Do they have the same biological activities? • the cloned v-src gene transforms cells • c-src has low transforming ability
Background image of page 6
Src is “myristylated” at its amino terminus • covalent attachment of myristylate (C14 fatty acid) • the lipid anchors Src to the plasma membrane Plasma membrane inside outside
Background image of page 7

Info iconThis preview has intentionally blurred sections. Sign up to view the full version.

View Full DocumentRight Arrow Icon
Brugge and Erikson, 1977: • Induced tumors in mice with an RSV derivative that was able to infect mammalian  cells  • They expected the mice would make antibodies to v-Src •  Isolated an antibody that could immunoprecipitate a 60 kD protein expressed only in 
Background image of page 8
Image of page 9
This is the end of the preview. Sign up to access the rest of the document.

Page1 / 31

Class 9 Srcprotein - Src, the protein Reading: The Hunting...

This preview shows document pages 1 - 9. Sign up to view the full document.

View Full Document Right Arrow Icon
Ask a homework question - tutors are online