pp_lab_report_enzyme.pdf - LABORATORY REPORT Activity...

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LABORATORY REPORT Activity: Enzyme Activity Name: Crisitna Westberry Instructor: Dr. Steelmen Date: 03.26.2018 Predictions 1. Sucrase will have the greatest activity at pH 3 2. Sucrase will have the greatest activity at 30 °C (86 °F) 3. Sucrase activity increases with increasing sucrose concentration until a plateau is reached Materials and Methods Effect of pH on Enzyme Activity 1. Dependent Variable amount of product (glucose and fructose) produced 2. Independent Variable pH 3. Controlled Variables temperature, amount of substrate (sucrose) present, sucrase + sucrose incubation time Effect of Temperature on Enzyme Activity 1. Dependent Variable amount of product (glucose and fructose) produced 2. Independent Variable temperature 3. Controlled Variables pH, amount of substrate (sucrose) present, sucrase + sucrose incubation time Effect of Substrate Concentration on Enzyme Activity 1. Dependent Variable amount of product (glucose and fructose) produced 2. Independent Variable amount of substrate (sucrose) present 3. Controlled Variables temperature, pH, sucrase + sucrose incubation time 4. Describe what is measured as an indicator of sucrase activity and why this is an indicator of sucrase activity. Amount of product (glucose and fructose) produced is what is being measured for this lab. This is an indicator of sucrase activity because the amount of product produced is a result of the experiment 5. Explain why denatured sucrase was used as a control. Laboratory Report/ Crisitna Westberry/ Enzyme Activity/ Dr. Steelmen/ 03.26.2018/ Page [1] of [4]
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because activated sucrase would increase the activity.
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