FOV and DA Problems 1.docx

FOV and DA Problems 1.docx - NAME FOV and DA Problems 1...

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NAME_____________________________ FOV and DA Problems 1 Day/Time________________________________ When you look through a microscope, you are looking at a certain area. We call this the “field of view” (fov). For example, at 40X (total magnification), your field of view may look like this: Note that at higher magnifications, your fov will contract, that is, less area is observed: We can directly measure the fov at scanning power by measuring it with a ruler. Procedure: Take a ruler, and on a small piece of paper (about 30 mm x 30 mm) trace 10 mm from the ruler. Get a blank slide and cover slip. Put the paper on the center of the slide and cover with a cover slip. Observe through the microscope at 40X. Count the number of mm covered by the diameter across the fov as shown
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above. You may observe more or less than shown. Record. Your are done! Return the slide and the cover slip to their boxes. Everything else you do will be done by calculation. Go to page 3 of the “Chromatic and Spherical Aberration” hand out. Use the formula shown to calculate the fov at 100X, 400X, and 1,000X.
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