2017 Microtech Hour Exam 2.docx - ANIMAL MICROTECHNIQUES...

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1 ANIMAL MICROTECHNIQUES AND TISSUE CULTURE Hour Exam #2 31 March, 2017 Name:__________________________ Be sure to read the questions carefully before answering. Keep your answers SHORT and to the point: there is no need to write a major essay for any of these questions! GOOD LUCK! 1. BRIEFLY describe the rationale for doing transfection experiments in mammalian cells. (15pt) 2. Briefly describe how you would go about isolating rat hepatocytes. (15pt)
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2 3. You’ve walked into the lab on Monday morning prepared to change the media on your cells. Upon opening the incubator you notice that several of your culture dishes are sporting fuzzy gray floating rafts, and when you look at the media on the microscope you observe numerous thread-like structures. What happened over the weekend? What should you do, and not do, now? (15 pt) 4. What characteristics of collagenase often make it a better choice for isolating primary cells than trypsin? (10pt) 2
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5. You have recently started to work for a small biotech start-up company here in New Jersey. The
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