fish fraud - Results Table 1: The SDS-PAGE set up of the...

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Results Table 1: The SDS-PAGE set up of the fish samples Lane Volume (micro litre) Sample 1 10 Kaleidoscope 2 10 Salmon 3 10 Trout 4 10 Scallop 5 10 Mom’s Salmon 6 10 Actin Myosin Table 1 describes the order in which the fish samples were placed after they had been treated with Laemmli sample buffer and heated at 95 o C. Table 2: +/- Matrix with the distance travelled in mm by each protein present in the gel Distance Traveled (mm) Kaleidoscop e Salmon Trout Scallop Mom’s Salmon Actin Myosin 13.5 + + + + + + 15.25 + - - - - - 16 - + + + + + 18 + - - - - - 20 - + + - + - 23 - + + + + - 24.5 - + + - + + 26.5 + + + + + + 28 + + + - + + 30 - + - - - -
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32 + + + + + - 33 + + + + + - 35 + - + - + + 38 + + + - + - 40 - + + + + + 42 - + + - + - 44 + - - + - - 46 - - + - + + 49.5 - + + - + - 52.5 + - + - + - Table 2 shows the distance travelled by each protein present in the gel as well as in the fish, and it also shows which protein is present in which fish sample. Since the proteins separate by weight, it is obvious that the heaviest proteins travelled the least distance because it is harder for them to travel a long distance, and vice-versa. This table can also be used to compare different fishes to see which fishes have the most proteins in common, hence determining which kind of fish “Mom’s Salmon” really is. By simple observation, it is clear that the Mom’s Salmon resembles the Trout most closely. Table 3: Percent Commonality between the different fish samples
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This note was uploaded on 03/21/2008 for the course BS 111L taught by Professor Alison during the Spring '08 term at Michigan State University.

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fish fraud - Results Table 1: The SDS-PAGE set up of the...

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