dog sperm part III - Cryopreservation of Epididymal Dog...

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Cryopreservation of Epididymal Dog Sperm Stephen Kramer Animal Science Wednesday A Lab
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Introduction Cryopreservation is a process in which cells or whole tissues, sperm cells in this case, are preserved by cooling to sub-zero temperatures. Cryopreservation was carried out with sperm saved from the cauda epididymus and vas deferens of domestic dog testes in this study. Epididymal sperm freezing has had limited success in specific species of cat (Goodrowe and Hay, 1993); pig: (Nagai et al., 1988), and cattle: Watson (1978). In humans, with the use of epididymal sperm, oocytes have been fertilized and subsequent pregnancies have occurred successfully (Friedler et al. 1998). Dog sperm can be stored for several months and be used later with some success in fertility (Marks et al., 1994). Discrepancies between the shape and size of spermatozoa among various canine breeds at different stages of sperm maturation can allow for more water damage during the freeze- thaw process (Watson 2000). The objective of this study was to obtain a successful technique to cryopreserve epididymal dog sperm by modifying current methods that have already proven that they can retain the sperm’s functional ability.
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This essay was uploaded on 12/11/2007 for the course AN SC 150 taught by Professor Butler,wr during the Spring '06 term at Cornell University (Engineering School).

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dog sperm part III - Cryopreservation of Epididymal Dog...

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