{[ promptMessage ]}

Bookmark it

{[ promptMessage ]}

Lab 4 Triston Wilson_CLW Comments.docx

Lab 4 Triston Wilson_CLW Comments.docx - Separation of...

Info icon This preview shows pages 1–2. Sign up to view the full content.

View Full Document Right Arrow Icon
Separation of Fluorene and Fluorenone through the Use of Column Chromatography Triston Wilson - [email protected] Abstract: The purpose of this lab was to separate a crude mixture of fluorene and fluorenone by column chromatography in order to discover how much of each were in the mixture. Dichloromethane and toluene was added to a mixture of fluorene and fluorenone, and then it was poured into a column packed with alumina and hexane. The column was drained into fractions that were then spotted for TLC in order to determine which fractions were fluorene or fluorenone. Fractions containing like substances were combined. Then, the solvent was boiled off in order to find the weight of each component and % recovery was calculated. Fractions 1-8 all shared similar Rf values which ranged from .563 to .614, and fractions 9-11 all had the exact same Rf value of .057. It was deduced that fractions 1-8 had fluorene in them because it is a non- polar molecule, and therefore moved across the silica plate much more than the polar molecule of fluorenone. Although separation was successful, only 34.0% of fluorene and 43.5% of fluorenone was recovered, so only 77.5% of the fluorene and fluorenone was recovered in all. This percentage could have been corrected if we had taken more fractions from the column in order to recover all of the fluorenone. Introduction: Column chromatography is one of the most useful techniques for separating and purifying compounds on a microscale. Column chromatography follows many of the same principles as TLC. It is also adequate for removing impurities closely related in structure.
Image of page 1

Info icon This preview has intentionally blurred sections. Sign up to view the full version.

View Full Document Right Arrow Icon
Image of page 2
This is the end of the preview. Sign up to access the rest of the document.

{[ snackBarMessage ]}

What students are saying

  • Left Quote Icon

    As a current student on this bumpy collegiate pathway, I stumbled upon Course Hero, where I can find study resources for nearly all my courses, get online help from tutors 24/7, and even share my old projects, papers, and lecture notes with other students.

    Student Picture

    Kiran Temple University Fox School of Business ‘17, Course Hero Intern

  • Left Quote Icon

    I cannot even describe how much Course Hero helped me this summer. It’s truly become something I can always rely on and help me. In the end, I was not only able to survive summer classes, but I was able to thrive thanks to Course Hero.

    Student Picture

    Dana University of Pennsylvania ‘17, Course Hero Intern

  • Left Quote Icon

    The ability to access any university’s resources through Course Hero proved invaluable in my case. I was behind on Tulane coursework and actually used UCLA’s materials to help me move forward and get everything together on time.

    Student Picture

    Jill Tulane University ‘16, Course Hero Intern