Lab 4 Triston Wilson_CLW Comments.docx

Lab 4 Triston Wilson_CLW Comments.docx - Separation of...

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Separation of Fluorene and Fluorenone through the Use of Column Chromatography Triston Wilson - [email protected] Abstract: The purpose of this lab was to separate a crude mixture of fluorene and fluorenone by column chromatography in order to discover how much of each were in the mixture. Dichloromethane and toluene was added to a mixture of fluorene and fluorenone, and then it was poured into a column packed with alumina and hexane. The column was drained into fractions that were then spotted for TLC in order to determine which fractions were fluorene or fluorenone. Fractions containing like substances were combined. Then, the solvent was boiled off in order to find the weight of each component and % recovery was calculated. Fractions 1-8 all shared similar Rf values which ranged from .563 to .614, and fractions 9-11 all had the exact same Rf value of .057. It was deduced that fractions 1-8 had fluorene in them because it is a non- polar molecule, and therefore moved across the silica plate much more than the polar molecule of fluorenone. Although separation was successful, only 34.0% of fluorene and 43.5% of fluorenone was recovered, so only 77.5% of the fluorene and fluorenone was recovered in all. This percentage could have been corrected if we had taken more fractions from the column in order to recover all of the fluorenone. Introduction: Column chromatography is one of the most useful techniques for separating and purifying compounds on a microscale. Column chromatography follows many of the same principles as TLC. It is also adequate for removing impurities closely related in structure.
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