Metabolism lab

Metabolism lab - Sandeep Bhateja 903600684 Life Sciences 2...

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Sandeep Bhateja 903600684 Life Sciences 2 Section 2F TA: Natalie 2-9-09 The Effect of Light on Metabolic Rate in Goldfish Introduction Metabolism is defined as the sum of all chemical reactions in an organism. These chemical reactions are necessary for the survival of an organism and control the way the organism lives its life. Thus, the ability to alter the rate of these reactions (metabolic rate) is of great interest to not only scientists, but all living creatures. Organisms acquire the energy required for these metabolic reactions through respiration, a process in which organisms obtain oxygen. In this lab we will monitor the respiratory rate of goldfish in a closed environment, which is correlated to metabolic rate. The goal of this experiment is to determine if a change in ambient light changes the oxygen consumption rate in goldfish. This will be accomplished by measuring the change in oxygen concentration per unit time. Our null hypothesis is that goldfish exposed to an increased or decreased amount of light will have no difference in metabolic rate compared to goldfish in ambient light. Materials and Methods Experimental materials include: Balance Thermometer
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Chamber with oxygen concentration sensor Goldfish (same species) Paraphernalia for collecting goldfish (nets, beakers, dish bins) Lamp Black drop cloth LoggerLite computer program Two goldfish will be used per trial. There will be 6 groups testing bright light versus ambient light, and 6 more groups testing dark environments versus ambient light. The control group is the pair of fish that are not exposed to differing ambient light levels; e.g. the default ambient light level. Data is recorded via the oxygen sensor chamber and LoggerLite software. The sampling rate is every 20 seconds, and the total length of one time trial is 10 minutes. A paired t-test will be used to analyze the data. The experiment begins with testing the control group, involving no change in ambient light. Prepare the chamber by filling it with “fish water”, which contains the correct pH level, salinity, and other factors that are necessary for the goldfish to survive. Calibrate the dissolved oxygen probe. The device must be warmed up for at least 10 minutes
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Metabolism lab - Sandeep Bhateja 903600684 Life Sciences 2...

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