LS lab 2 photosynthesis results

LS lab 2 photosynthesis results - Results In experiment 1,...

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Results In experiment 1, the results of pigment composition of the dark-grown barley are taken alongside the light-grown barley. First of all, we looked at the control group, the normal light-grown barley. The TLC strip of light barley showed a complicated separation pattern with the following colours and retention factors: orange, 1; dark green, .875; light green, .6875; yellow, .5; and yellow, .3125. The light-grown barley also showed a complex spectrophotometry spectrum of absorption, with peaks centered from 400 to 700nm. The two strongest peaks are shown at wavelengths of 440nm—a very thick peak suggesting it is made up of many smaller peaks—and 670 nm (see Graph 1). This data is then compared to that of dark-grown barley. The TLC strip of dark barley had a simpler pattern with the two following colours and retention factors: light orange with an Rf of 1 and yellow with an Rf of .625. Dark-grown barley showed a spectrophotometry spectrum that fell mostly in the 400nm region. Although not exceeding clear, the results show absorption at 415 nm, 430 nm, and 440 nm (as seen in Graph 2). In experiment 2, evolutionary history is found by comparing the results of pigment composition of light-grown barley to that of four other organisms: green algae, brown algae, red algae, and cyanobacteria. Green algae showed a TLC spotting with colours and retention factors: orange, 1;
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LS lab 2 photosynthesis results - Results In experiment 1,...

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