Sp09MicroLab1Microscopy - Bacillus cereus The smear was...

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University of Texas at Austin BIO126L Course Instructor: Pratibha Saxena Spring 2009 Lab 1: Microscopy Lab Purpose To learn how to properly utilize a light microscope and to understand magnification and resolution of the light microscope through observation of various cell types with the different objective lenses. Discussion The use of multiple cell types for observation at different magnifications was very helpful to see how much of a difference each objective actually made in the ability to observe cells. The difference between the 10x and 40x objectives was much greater than that between the 40x and 100x objectives. Even with the largest of the observed cells (the epithelial cells) the 10x did not adequately show detail of this cell type. There was also a marked difference between wet slides in comparison with the dry, smear slide (of
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Unformatted text preview: Bacillus cereus ). The smear was much easier to observe at different magnifications because the cells did not move around as the wet slide cells did. As a result, on the wet slides, I am not sure that the same object was observed at the different magnifications. Overall this lab was very beneficial to learn the basic use of the microscope and to observe different cell types and this experience will be very valuable in the future. Therefore, I believe that all aspects of the purpose of this lab were met. Assigned Questions Q3) The immersion oil serves to focus more of the light from the specimen through the objective lens so that the specimen can be seen in better detail. Water would not be a very good substitute because its refractive index is not close to that of glass (1.52), as is the case of the immersion oil....
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