Sp09MicroLab3SelectionDifferentiation - observed...

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University of Texas at Austin BIO126L Course Instructor: Pratibha Saxena Spring 2009 Lab 3: Selection and Differentiation Purpose To observe the effects of environmental conditions on the growth of microbial organisms, through organism interactions, antibiotic sensitivity, oxygen tolerance and pH tolerance. To observe selection and differentiation through the use of varying mediums. Discussion This lab was valuable in showing how certain environmental conditions affect organisms and more importantly that these conditions can be used for selection and differentiation. The microbial interactions experiment showed how other species of microorganism can effect the growth of another. As stated in the lab manual, the
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Unformatted text preview: observed interactions are examples of commensalisms whereas the antibiotic sensitivity (if the antibiotic was produced directly by another organism near the test organism instead of on disks) would be considered antagonism. Ultimately, these growth inhibitors or promoters can be valuable to select one organism out of many (selection). The MacConkey Agar plates, also, adequately demonstrated selection due to the presence of crystal violet and bile salts, which select against gram positive organisms. It also differentiates which organisms ferment lactose because it contains neutral red....
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This note was uploaded on 04/27/2009 for the course BIO 126L taught by Professor Saxena during the Spring '08 term at University of Texas.

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