Sp09MicroLab10Pathogens - University of Texas at Austin...

Info iconThis preview shows page 1. Sign up to view the full content.

View Full Document Right Arrow Icon
University of Texas at Austin BIO126L Course Instructor: Pratibha Saxena Spring 2009 Lab 10: Pathogenic Bacteria Purpose To determine the identity of 3 unknown, mildly pathogenic bacteria using selective media and biochemical tests. Discussion If the KIA slant was made incorrectly with 1% glucose as well as 1% lactose then the organisms would not use up the glucose as quickly so results obtained at 18-20hrs would be incorrect (the slant and butt would be yellow for all of the organisms). Gram - rods grown on MacConkey plate (to select against gram + organisms): The KIA slant was very helpful in determining the identity of my unknown enterics. My enteric #1 had a yellow butt and slant and had produced a large amount of gas at 18-20hrs indicating this organism to be a coliform aerogenic fermenter. After 20hrs, the slant turned red/pink because the organism had started to metabolize peptones. My enteric #2
Background image of page 1
This is the end of the preview. Sign up to access the rest of the document.

This note was uploaded on 04/27/2009 for the course BIO 126L taught by Professor Saxena during the Spring '08 term at University of Texas.

Ask a homework question - tutors are online