MicroLab4DilutionPlating

- are multiple ways of getting the same amount of dilution The results of the dilution plate showed very distinctly the differences in the F G and

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Inc # 204 Heather Oslund BME 2.106 Th 2-5 WonYoung 2-26-09 Lab 4: Dilution and plating Purpose To observe and learn how to complete and calculate dilutions through test tube and plate dilution processes. To learn how to properly use micropipettors and spectrophotometers. To learn about colorimetry and turbidimetry and how they are used bacteriologically. Discussion In the dye dilution experiment test tube A looked the most concentrated, very dark red. This is supported by the calculation that tube A had a concentration of 10 u/ml, which was higher than any of the other test tubes. Test tubes C and Z were both a very light pink color and they turned out to, both, have the lowest dilution at 0.1 u/ml showing that there
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Unformatted text preview: are multiple ways of getting the same amount of dilution. The results of the dilution plate showed, very distinctly, the differences in the F, G, and H samples. The plates for the stock, B, and D tubes looked very similar to each other with no distinct colonies. Although there were too many colonies to count (over 300), on the plates for sample F, you could make out distinct colonies. Plate G had an average of 38 colonies per plate so that was the one used to calculate the original titer and plate H did not have enough average colonies (<10). At an O.D. of 0.6 the titer would be 3.59 x 10 7 CFU/ml....
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This note was uploaded on 04/27/2009 for the course BIO 126L taught by Professor Saxena during the Spring '08 term at University of Texas at Austin.

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