Prob set 2 - Problem Set 2 General Comments on Problem Set...

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Problem Set 2 General Comments on Problem Set 1: Some of you need to explain your answers in a bit more detail. Chapter 10 problems: Solved Problems at the end of this chapter: Please review all of the solved problems. Questions: 1. DNA is in the form of a double helix and is thus coiled. Supercoiling is coiling of an already coiled structure. a) What is the difference between positive and negative supercoiling? b) which bacterial enzyme increases negative supercoiling and which can relax negative supercoils? c) what is the general mechanism of action of those enzymes? d) how do the quinolone type of antibiotics affect bacteria? 2. a) Please define denaturation and renaturation in your own words. b) Will there be a difference in the rate of denaturation of the following two sequences? Please explain. c) Will there be a difference in the rate of renaturation rate? Please explain. d) suppose that sequence 1 is present in many, many copies, while sequence 2 is present in only a few copies in an organisms genome. Will denaturation rate be affected by the difference in
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Prob set 2 - Problem Set 2 General Comments on Problem Set...

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