Problem Set 3 Chapter 11 and part of 18 problems: Solved Problems at the end of the chapter: Please review solved problems 1, 3 and 4. You do not need to turn in anything for them, but it is good to review them. Questions: 1. a) Please describe the characteristics that all DNA polymerases share. b) what is an important characteristic that most have but others lack? c) Please compare and contrast the overall structure and functions of DNA polymerase III and DNA polymerase I in prokaryotes. Be specific. 2. Please describe the role of DNA polymerase III in synthesis of the lagging strand. How is this different from synthesis of the leading strand? 3. DNA synthesis in vivo and in polymerase chain reaction (PCR): a) Please describe the process of strand separation in prokaryotes at the origin of replication. b) PCR takes place in small tube with DNA that will serve as the template for DNA synthesis. How is strand separation accomplished? What other important proteins that are necessary for
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This note was uploaded on 04/27/2009 for the course BIO 340 taught by Professor Steele during the Spring '09 term at Arizona.