Problem set 7

Problem set 7 - Problem set 7 Chapter 18 Review solved...

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Problem set 7 – Chapter 18 Review solved problems 1, 2 and four at the end of chapter 18. Please review but do not turn in, problems E9, E13, E20, E23 E31 and E32. 1. Restriction endonucleases (restriction enzymes): What is the source of all these enzymes and what is their role outside the lab? What is a restriction site? Write out two different restriction sites indicating both strands (see table 18.3 or another text or an on-line source). Restriction site mapping: Use the information in the linear restriction map shown below to answer the following questions. B and E indicate the restriction sites for Bam HI and Eco RI respectively and the numbers indicate the number of kb from one end of the DNA molecule to near the other end (each division represents one kb in length). a) Draw a diagram of a gel that indicates the results from using restriction enzyme B to cut this fragment of DNA. b) Draw a gel that indicates the results from using both restriction enzymes at the same time to cut this fragment of DNA. Be sure to
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Problem set 7 - Problem set 7 Chapter 18 Review solved...

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