Quiz 2keynew - Quiz 2 Key, Biology 340, General Genetics,...

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Quiz 2 Key, Biology 340, General Genetics, Spring, 2008 1. a) please briefly explain what happens when DNA is denatured. b) what characteristic of the DNA may affect the rate [should say temperature not rate] at which denaturation occurs? c) please briefly explain the process of renaturation of DNA. d) If fragments of genomic DNA are renatured what might affect the rate of renaturation of that DNA? a) when DNA is heated the hydrogen bonds between the two strands break and the two strands will separate. b) the base composition of the DNA will affect the temperature at which denaturation occurs. For example, a fragment of DNA that is 60% G/C and 40% A/T will need a higher temperature to denature than will a similar-sized fragment of DNA that is 40% G/ C and 60% A/T. [Rate is affected by the length of the fragment.) c) renaturation occurs when the DNA is cooled. Renaturation is based on the formation of hydrogen bonds between complementary base pairs. d) copy number of the DNA [for example, high copy number = repetitive DNA] will affect the rate at which renaturation occurs. The more copies of a particular fragment that
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This note was uploaded on 04/27/2009 for the course BIO 340 taught by Professor Steele during the Spring '09 term at University of Arizona- Tucson.

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Quiz 2keynew - Quiz 2 Key, Biology 340, General Genetics,...

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