Quiz 3key

Quiz 3key - 1 Quiz 3 Biology 340 General Genetics Spring...

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Quiz 3, Biology 340, General Genetics, Spring, 2008 1. Polymerase chain reaction (PCR): a) what is the overall or basic goal of PCR? b) what are the main components that are present in each tube and what is the function of each? a) the goal is to produce many copies of a specific region of DNA b) template DNA – this includes the region of interest; dNTP’s (what does N stand for?) – these are the nucleotides that are added to the new strands of DNA that are being synthesized; DNA primers that flank the region of interest; taq polymerase – the thermostable DNA polmerase that synthesizes the DNA. 2. List the three steps of each PCR cycle, the approximate temperature at which it occurs and what happens in each step. 1) denaturation of dsDNA; 95 degrees Celsius 2) primer annealing; 45 – 65 degrees Celsius (Why is there a range of variation of this temperature? Why might a low temperature be used? What might be an advantage of the higher temperature? 3)
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This note was uploaded on 04/27/2009 for the course BIO 340 taught by Professor Steele during the Spring '09 term at Arizona.

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Quiz 3key - 1 Quiz 3 Biology 340 General Genetics Spring...

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