Lecture 2 - Antigen Discovery

Lecture 2 - Antigen Discovery - ANTIGEN DISCOVERY Edwin...

Info iconThis preview shows pages 1–10. Sign up to view the full content.

View Full Document Right Arrow Icon
EVO ANTIGEN DISCOVERY Edwin Oaks, Ph.D. George Mason University Fall 2008
Background image of page 1

Info iconThis preview has intentionally blurred sections. Sign up to view the full version.

View Full DocumentRight Arrow Icon
EVO Antigen Discovery, Definitions Antigen: a foreign substance that when introduced into the body stimulates a specific immune response Antibody: a protein produced by B-cells that binds specifically to an antigen Proteomics: the analysis of the expression, localization, functions, and interactions of the proteins expressed by the genetic material of an organism Genomics The study of genes and their function, includes mapping and sequencing DNA. American Heritage Dictionary
Background image of page 2
EVO Antigen Discovery, Definitions SDS-PAGE: sodium dodecyl sulphate - polyacrylamide gel electrophoresis. Separates proteins based on size. 2-D gel electrophoresis: High resolution technique for separating proteins, first by isoelectric focusing then by SDS- PAGE Western blot: A technique for identifying specific antibodies or proteins in which proteins are separated by SDS-PAGE, transferred to nitrocellulose, and reacted with antibody American Heritage Dictionary
Background image of page 3

Info iconThis preview has intentionally blurred sections. Sign up to view the full version.

View Full DocumentRight Arrow Icon
EVO Bacterial Cell Structure
Background image of page 4
EVO Crucial to pathogenesis of agent Accessible to immune effectors Secreted Toxins Enzymes Located on surface LPS Pili, flagella, capsules, invasins Viral glycoproteins Capsid proteins of viruses Selection of vaccine candidate antigens
Background image of page 5

Info iconThis preview has intentionally blurred sections. Sign up to view the full version.

View Full DocumentRight Arrow Icon
EVO Selection of vaccine candidate antigens Recognized during natural infection/disease It is an antigen Immune response to target is correlated with protection Antigen is found in all pathogenic serotypes/species of the pathogen Offers broad immunity, need fewer vaccines “conserved”
Background image of page 6
EVO Antigen Discovery: Techniques Antibody based techniques Use serum from infected individuals Require source of pathogen for laboratory analysis Easy for cultivatable pathogens Difficult for some pathogens Certain viruses Rickettsial agents New pathogens
Background image of page 7

Info iconThis preview has intentionally blurred sections. Sign up to view the full version.

View Full DocumentRight Arrow Icon
EVO Antigen Discovery: Techniques, 2 Early antigen discovery depended on purification and biochemical characterization Directly from agent Required ability to produce large quantities of agent Often based on “crude” physical properties » Heat sensitive or stable Depended on “visual” reaction with antisera » Agglutination » Hemolysis » Complement fixation » Plaque neutralization
Background image of page 8
EVO Antigen Discovery Techniques, 3 Modern techniques Immunoprecipitation Antibodies + radiolabeled antigen » Forms complex » Collected by protein A » Run on SDS-PAGE
Background image of page 9

Info iconThis preview has intentionally blurred sections. Sign up to view the full version.

View Full DocumentRight Arrow Icon
Image of page 10
This is the end of the preview. Sign up to access the rest of the document.

This note was uploaded on 04/29/2009 for the course BIOL 420 taught by Professor Edwardsoaks during the Fall '08 term at George Mason.

Page1 / 31

Lecture 2 - Antigen Discovery - ANTIGEN DISCOVERY Edwin...

This preview shows document pages 1 - 10. Sign up to view the full document.

View Full Document Right Arrow Icon
Ask a homework question - tutors are online