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Unformatted text preview: Absorption Spectrum Anam Ahmad CHEM 211-204 4/22/09 Lab Report Purpose: To determine the wavelengths of visible light absorbed by substances in solution and plot an absorbance spectrum. Procedure: 1. prepare 5 standard solutions (0, 3, 5, 7, and 10 ml of stock solution) 2. set the spectrometer to max and calibrate instrument 3. get transmittance for each of the 5 standard solutions 4. convert transmittance to absorbance by using abs = -log(%T/100) 5. plot concentration vs. absorbance 6. get unknown 7. set max 8. obtain transmittance and convert to absorbance Results: RAW DATA Explanation: o Lambda_max: wavelength of maximum absorption o Conc_stock: concentration of stock solution o Vi_A: volume of stock solution in standard solution 2 o Vf_A: diluted volume of solution 2 o Vi_B: volume of stock solution in standard solution 3 o Vf_B: diluted volume of solution 3 o Abs_stock: absorbance of stock solution o Abs_A: absorbance of standard solution 2 o Abs_B: absorbance of standard solution 3 o Abs_water: absorbance of water o Abs_unknown: absorbance of unknown solution metal Lambda_ max Conc_ stock Vi_A Vf_A Vi_B Vf_B Abs_ stock Abs_A Abs_B Abs_ wate r Abs_ unknown Cobalt 515 0.2 3 10 7 10 12.212....
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