ps2q - MIT Department of Biology 7.014 Introductory...

Info iconThis preview shows pages 1–4. Sign up to view the full content.

View Full Document Right Arrow Icon
MIT Department of Biology 7.014 Introductory Biology, Spring 2004 Name:__________________________________________ Section: ______ 7.014 Problem Set 2 Please print out this problem set and record your answers on the printed copy. Problem sets will not be accepted late. Question 1 A schematic of the active site of an enzyme with its substrate bound is shown below. The side chains of the amino acids important to the function of that active site are shown as a-e. _ CH 3 H O C CH 2 a C N O C C NH O e CH 2 C NH 2 2 N C CH 3 O C C O HO CH b C C CH 3 CH 3 CH 3 CH 3 CH 3 CH 3 CH 2 CH CH CH 2 d c a) Identify each of the amino acids a-e and indicate the strongest type of non-covalent interaction it makes with the substrate. # Amino acid Interaction with substrate a b c d e 1
Background image of page 1

Info iconThis preview has intentionally blurred sections. Sign up to view the full version.

View Full DocumentRight Arrow Icon
Name:__________________________________________ Section: ______ Question 1, continued The isolated substrate for this enzyme is shown below (rotated 90 ° to the left). For parts b) and c) below ignore size considerations. NH 2 O C C C N C H CH 3 C N C C C CH 3 CH 3 O b) For each of the following, indicate if the interaction with the enzyme would be stronger than that seen with the original substrate, the same as that seen with the original substrate, or weaker than that seen with the original substrate. For each, explain your reasoning. i) NH 2 O interaction (circle one): stronger weaker same CH 3 reasoning: N C C C C H C N C CH 3 C C CH 3 CH 2 O CH 3 ii) NH 2 O interaction (circle one): stronger weaker same CH 3 C C C H + reasoning: N C C N C CH 3 C C CH 3 O iii) NH 2 O interaction (circle one): stronger weaker same CH 3 H C C reasoning: N C C N C C C C CH 3 CH 3 SH 2
Background image of page 2
Name:__________________________________________ Section: ______ Question 1, continued c) The following modified substrate does not bind. You want to change one amino acid of the enzyme active site to allow this version of the substrate to bind. Your change should maximize the strength of the interaction between the substrate below and the enzyme. O _ NH 2 O H C C C O N C C C N C C C CH 3 CH 3 O i) Which amino acid would you change? ii) To what would you change it and why?
Background image of page 3

Info iconThis preview has intentionally blurred sections. Sign up to view the full version.

View Full DocumentRight Arrow Icon
Image of page 4
This is the end of the preview. Sign up to access the rest of the document.

Page1 / 8

ps2q - MIT Department of Biology 7.014 Introductory...

This preview shows document pages 1 - 4. Sign up to view the full document.

View Full Document Right Arrow Icon
Ask a homework question - tutors are online