ps3q - MIT Department of Biology 7.014 Introductory...

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7.014 Introductory Biology, Spring 2004 Name:__________________________________________ Section: ______ 7.014 Problem Set 3 Please print out this problem set and record your answers on the printed copy. Problem sets will not be accepted late. Question 1 Hypothetical organism X has the following DNA sequence. Part of the promoter is indicated by the boxed sequence. Transcription starts at and includes the bold A/T base pair. 5’ xxxx TATTTGAT A G CTCTATGCAT GCATGGGTCC TGAAGTTCAG ATCTTTGAGT CATAGGAGTC 3’ 3’ xxxx ATAAACTA T C GAGATACGTA CGTACCCAGG ACTTCAAGTC TAGAAACTCA GTATCCTCAG 5’ a) Give the RNA sequence of the first 25 bases following transcription. b) What are the first 5 amino acids of the resulting protein? c) Finish translating the following mRNA sequence. 1 5’ AUA UUU AUG CAU GGG ACU UAU AGC GAU AGC UAC UAA CAU AAG 3’ ile phe met ___ ___ ___ ___ ___ ___ ___ ___ ___ ___ ___ d) The organism that makes the above RNA sequence is exposed to a mutagen. What does a mutagen do? e) For each of the following mutations, identify the type of mutation (insertion, deletion, point, or silent) that occurred in the DNA and the affect on the resulting protein. Consider each mutation independently. i) C10
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This note was uploaded on 05/02/2009 for the course BIOL 7.014 taught by Professor Walker during the Spring '05 term at MIT.

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ps3q - MIT Department of Biology 7.014 Introductory...

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