quiz2ho - MIT Department of Biology 7.014 Introductory...

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Unformatted text preview: MIT Department of Biology 7.014 Introductory Biology, Spring 2005 7.014 Quiz II Handout **This will be a closed book exam** Question 1 Shown below is a representation of an origin of replication. 5' 3' 5' 3' A C B D GGAAC CAAGG GGAAC CAAGG fork 1 fork 2 template 4 template 3 template 1 template 2 ORI a) For the following, use sites A and B with respect to fork 1 and sites C and D with respect to fork 2. i) On which strand(s) will replication be continuous? template 1 template 2 template 3 template 4 ii) To which site or sites ( A , B , C , or D) can the primer 5'-GUUCC-3' bind to initiate replication? iii) When DNA ligase is inhibited, it differentially affects the synthesis from the leading and the lagging strands. Explain which strand (leading or lagging) is more affected by the lack of DNA ligase and why. 2 Question 1, continued b) The next nucleotide to be added to a growing DNA strand is dCTP (shown). Circle the part of the growing DNA chain to which the next base is attached. Circle the part of the dCTP that is incorporated into the growing DNA chain. N N NH 2 Growing DNA chain N N dCTP CH 3 O O O O- P O CH 2 O O P O O P O O -O-O -O C C C CH 2 C C C O C OH H C OH H c) DNA Replication involves many different enzymatic activities. Match each enzyme activity listed below with the function(s) that it has in the replication process. The first one is done for you. Enzyme Activity Function(s) Topoiosmerase k Primase (synthesizes primer) DNA polymerase to elongate new DNA strand Helicase to unwind DNA DNA polymerase to replace RNA with DNA Processivity factor Choose From: a) 3 5 growth of new DNA strand b) 5 3 growth of new DNA strand c) 3 5 exonuclease d) 5 3 exonuclease e) Makes RNA primer complementary to the lagging strand f) Makes RNA primer complementary to the leading strand g) Makes peptide bonds h) Separates the two DNA strands i) Maintains DNA polymerase on template j) Provides 3 hydroxyl for initiation of DNA polymerization k) Untangles super-coiled DNA 3 ___ Question 2 Below is the partial sequence of the sevenohwunforin ( 7014in ) gene, hypothesized to be mutant in students who take 7.013 Introductory Biology and in those students at the other school up the river. The promoter is underlined and transcription begins at and includes the bold G/C base pair....
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This note was uploaded on 05/02/2009 for the course BIOL 7.014 taught by Professor Walker during the Spring '05 term at MIT.

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quiz2ho - MIT Department of Biology 7.014 Introductory...

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